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DYKDDDDK-Tag (FLAG-tag) rabbit pAb

簡要描述:SourceRabbitApplicationsWB,ELISAReactivitySpeciesindependentDilutionWB:1:1000-3000;ELISA:1:20000

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  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2024-11-11
  • 訪  問  量:194

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詳細(xì)介紹

  • Source

    Rabbit

  • Applications

    WB,ELISA

  • Reactivity

    Species independent

  • Dilution

    WB: 1:1000 - 3000; ELISA: 1:20000. Not yet tested in other applications.

  • Storage

    -20°C/1 year

  • Specificity

    FLAG-tag Polyclonal Antibody detects FLAG-tagged recombinant proteins or FLAG-tagged proteins overexpressed in cells.

  • Source/Purification

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen

    DDDDK synthetic peptide conjugated to KLH.

  • Form

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.05% sodium azide.

  • Clonality

    Polyclonal

  • Background

    The DYKDDDDK (FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal FLAG vectors provide an Ek cleavage site for removal of the fusion tag. The FLAG peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the FLAG peptide is more likely to be accessible to antibodies. A FLAG-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on.

  • Concentration

    1 mg/ml

Product Reactivity Applications Conjugation Catalog Images

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