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TAP Tag mouse mAb(Mix)

簡(jiǎn)要描述:

  • 產(chǎn)品型號(hào):
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-26
  • 訪  問(wèn)  量:109

詳細(xì)介紹

  • Source

    Mouse

  • Applications

    WB

  • Reactivity

    Species independent

  • Dilution

    WB: 1:500-10000

  • Storage

    -20°C/1 year

  • Specificity

    The antibody detects TAP recombinant protein.

  • Source/Purification

    The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

  • Immunogen

    Recombinant Protein of TAP Tag

  • Form

    PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

  • Clonality

    Monoclonal

  • Background

    The TAP (Tandem Affinity Purification) method is an affinity purification method for the isolation of TAP-tagged proteins along with associated proteins. The TAP tag historically consists of a calmodulin binding peptide (CPB), a tobacco etch virus (TEV) protease cleavage site, and Protein A. However, additional tag combinations have been used with the TAP method including the combination of FLAG tags and HA tags. The TAP method permits the identification of proteins interacting with a particular target protein without any prior knowledge about the function, activity, or composition of the interacting proteins. The TAP tag has been especially useful and deployed with Yeast Tap-tagged ORF clones. These clones contain genomic fusions of the TAP construct and are extremely useful for determining natural protein interactions and expression level variations based on physiological changes.

  • Concentration

    1 mg/ml

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